IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Elevated number of circulating plasmablasts in patients with acute Hantavirus Pulmonary Syndrome caused by Andes virus infection
Autor/es:
GARCÍA M; MARTINEZ V; IGLESIAS A; LANDONI V; SASIAIN MC; SCHIERLOH P
Lugar:
Mar del Plata
Reunión:
Congreso; SAIC-SAI 2014; 2014
Resumen:
The presence of ?immunoblast-like? cells in patients with Hantavirus Pulmonary Syndrome (HPS) is a regular finding during routine clinical blood exam. Nonetheless their cellular linage has not been established nor their relevance in the course of the disease. In order to characterize the phenotype of those immunoblasts in HPS patients caused by the Andes virus (ANDV), anticoagulated blood samples were obtained from confirmed HPS patients (ANDV+ n=12), seronegative patients with acute respiratory symptoms (ARS n=12) and healthy subjects (HS n=21). Samples were inactivated and stabilized under biosecure conditions and processed for flow cytometry and confocal microscopy for quantitative (CountBright?) and qualitative analysis. In ANDV+ acute samples, we observed high numbers of CD19+ CD27hi CD38hi CD20neg IgDneg CD138+/neg intracellular Igshi blasted (FSChiSSChi) lymphoid cells identified as circulating plasmablasts (PB), a B cell-derived population virtually absent on HS (p