Differences in long non-coding RNA expression associated with high grade histological variants of follicular lymphomas are related with aggressiveness-deregulated pathways either in tumor as in microenvironment compartments

ALEJANDRO ROISMAN; ALBA NAVARRO; MIRIAM PRIETO; CONCEPCIÓN MUÑOZ; GUILLEM CLOT; ANA BELÉN LARQUE; MARÍA ADELA SACO; ANTONIO MARTÍNEZ; MARTA KULIS; ANA QUEIROS; JOSE IGNACIO MARTÍN-SUBERO; ALBA MATAS; PATRICIA PÉREZ; ANNA ESTEVE; MARC DABAD; SIMON HEATH; MONICA BAYES; MATTIA BOSSIO; PAU BELLOT; PHILIPPE SALAMBIER; ALBERT OLIVERAS; IRMA SLAVUTSKY; MARINA NARBAITZ; LAURA MAGNANO; EVA GINÉ; ANDREAS ROSENWALD; GERMAN OTT; MARTA AYMERICH; ARMANDO LÓPEZ GUILLERMO; ELÍAS CAMPO; LUIS HERNÁNDEZ

Barcelona

Workshop; 10th Workshop on Genomics and Proteomics; 2014

Societat Catalana de Biologia

Follicular lymphoma (FL) is a lymphoid neoplasm in which expression deregulation of coding genes either in tumor as in the microenvironment cells have been shown to be related with pathogenesis and patient prognosis. Nevertheless, the application of these results to the clinics is resulting difficult and, therefore, more powerful and consistent prognostic markers are still needed. Recently, expression levels of some long non-coding RNAs (lncRNAs) have been associated with the aggressiveness of several cancers including lymphomas and, therefore, they are interesting candidates for new biomarker discovery. In the present study we performed whole transcriptome profiling by RNAseq in 28 samples of FL with different histological grades either in tumor purified cells as in whole samples also containing the normal microenvironment. In both sample types we found differentially expressed (DE) coding transcripts and lncRNAs between high and low grade FL. Next, we used a Treelet-based algorithm to define those coding genes that were highly correlated with the DE lncRNAs found. The results showed that some DE lncRNAs were highly correlated with DE coding transcripts previously related with FL aggressiveness (proliferation in tumor cells and M2 macrophage polarization in the microenvironment cells). In summary, these results pointed to a subset of lncRNAs that could be pathogenetically relevant in FL and that constitute good candidates for biomarkers with clinical value that would be soon validated in larger series of patients.