IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Human naïve B cells are capable to phagocytose Mycobacterium Bovis-BCG in a lectin-assisted manner
Autor/es:
SCHIERLOH P; GARCIA MARINA; LANDONI VERÓNICA INÈS; KVIATCOVSKY, D; BALBOA L; SASIAIN MC
Reunión:
Taller; I Taller sobre Evasión Inmune Academia Nacional de Medicina; 2014
Resumen:
Mycobacterium tuberculosis complex (MTC) is a genetically related group of Mycobacterium species that cause tuberculosis in humans and other mammals. These intracellular pathogens invade Professional Antigen presenting cells (APC) where they subvert the phagosome maturation in order to avoid microbicidal reactions. Herein, by in vitro infection experiments conducted under different culture conditions; we search for leukocytes other than APC that may be susceptible to M.bovis-BCG (Pasteur strain) infection. Surprisingly, human naïve B cells ?but not NK nor T cells- were able to rapidly internalize live M.bovis-BCG by a cytochalasin D-sensitive mechanism. This process was entirely dependent on the pre-coating of bacteria with tetrameric Concanavalin A (Con A) in the presence of Ca2+ (p