Inflammation Induced By Cold Ischemia: A New Fast and Simple In Vivo Method to Evaluate Potential Therapeutic Tools N

AMBROSI, D. ; GUERRIERI,. ; BOUSO,; G. SALAMONE, ; J. MENDIBERRI, ; C. INCARDONA, ; E. CHULUYAN,.; CASADEI

Simposio; World Transplant Congress, Moscone West Convention Center; 2014

A prolonged cold ischemic (CI) time in kidney transplants has been associated with a high rate of DGF and reduced graft survival. The aim of this work was to develop a simple and rapid method to evaluate in vivo potential therapeutic tools, which may reduce the inflammatory process caused by the prolonged CI. Kidney explants (Exp) derived from BALB/c (WT) were incubated ex vivo at 4 ° C for 18 h in different preservation solutions. At the end of the incubation period, explants were implanted subcutaneously (s.c.) in WT or transgenic mice expressing green fluorescent protein (GFP). As control group and, at the same time, these animals also received explants that do not suffered CI. The recruitment of neutrophils was examined after 24 hours by measuring myeloperoxidase (MPO), as an indirect marker of neutrophils migration, and by a direct counting of the Gr1-Phe+ and GFP+ cells with a flow cytometer. Explants that were incubated at 4ºC for 18 h in Ringer Lactate (RL), recruited more neutrophils than control explants (p