NEUTROPHILS ENHANCE GAMMA DELTA T CELL ACTIVATION TRIGGERED BY ANTI-CD3 ANTIBODIES.

TOWSTYKA N; BORGE M; SABBIONE F; GIORDANO M; GEFFNER J; TREVANI AS; JANCIC C

Mar del Plata

Congreso; LXII Reunión Anual de la Sociedad Argentina de Inmunología; 2014

gd T cells act as early sensors of cellular stress and infection; they use both, the TCR and additional activating receptors (i.e. NKG2D, TLRs), to respond to stress and/or infection-induced ligands. Furthermore, gd T cells play an important role in linking the innate and adaptive immunity. gd T lymphocytes modulate the function of neutrophils; and growing evidence supports a critical role of gd T cells in the recruitment and activation of neutrophils at the sites of infection. We previously reported that neutrophils suppress gd T cell activation induced by the specific agonist HMBPP, in a ROS-dependent manner. Now, we analyzed whether neutrophils were able to modulate the phenotype and function of human blood gd T cells activated by anti-CD3 antibodies. For this purpose, we analyzed the expression of T cell activation markers, CD25 and CD69 by flow cytometry and the production of IFN-g by ELISA. In this study, we found that the up-regulation of CD25 (p