IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
PLANT HEAT SHOCK PROTEIN 90 AS CARRIER-ADJUVANT FOR IMMUNIZATION AGAINST A REPORTER ANTIGEN
Autor/es:
MARIANA CORIGLIANO; FENOY I; SANDER V; MAGLIOCO A; GOLDMAN A; MARINA CLEMENTE
Lugar:
Los Cocos, Córdoba
Reunión:
Congreso; LXI Reunión Anual de la Sociedad Argentina de Inmunología; 2013
Institución organizadora:
SAI
Resumen:
The main goal of adjuvant research is to find new adjuvants able to stimulate Th1, Th2 or both responses but with reduced toxicity. In this context, the study of the immunomodulatory properties of molecules and proteins derived from different organisms can contribute to find new and more efficient adjuvants. Heat Shock Proteins (HSPs) such as Hsp60, Hsp70 and Hsp90 have been recently reported to play important roles in antigen presentation, activation of lymphocytes and macrophages, and maturation of dendritic cells. Therefore, plant Hsp90s are an attractive option to use as novel and interesting carriers-adjuvants for proteins or peptides of immunoprotective value. In the present study, we evaluated the modulation of the immune response induced by Hsp90 of Nicotiana benthamiana (NbHsp90.3) against the Maltose Binding Protein (MBP) as a reporter antigen. Equimolar quantities of recombinant proteins were administered in mice as follows: MBP alone (MBP group), a mixture of MBP and rNbHsp90.3 (MBP+rNbHsp90.3 group) and the fusion of MBP to rNbHsp90.3 (MBP-rNbHsp90.3 group). Although a significative increase of MHC class I surface molecules was observed in MBP+rNbHsp90.3 and MBP-rNbHsp90.3 groups, the covalent linkage between NbHsp90.3 and MBP to bring a fusion protein was essential to induce the strong specific antibody response with predominance of IgG2a. Eighty-four days after the first immunization, splenocyte proliferation from MBP-rNbHsp90.3-immunized mice was consistently higher than those from MBP and MBP+rNbHsp90.3 groups. In addition, splenocytes from MBP-rNbHsp90.3 immunized mice produced higher levels of IFN-gamma than controls. Finally, the percentage of CD8+ T cells significantly increased in MBP-rNbHsp90.3-immunized mice. Taken together, these results suggest that plant HSP90s could be incorporated as adjuvants in vaccines that require the generation of a Th1 response along with a CD8 cytotoxic cell response to confer immunity.