IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Humoral and cellular immune response to the hepatitis B virus vaccine in health care workers.
Autor/es:
HERNAN JULIN; ANDREA SALOMONE; NATALIA ALOISI; PATRICIA BARÉ; CARMEN CABELLO; CARMEN STANGANELLI; CLAUDIA FERRARIO; GOLDSTEIN DE FINK SUSANA
Lugar:
Los Cocos, Córdoba
Reunión:
Congreso; LXI Reunión Anual Sociedad Argentina de Inmunología.; 2013
Institución organizadora:
Sociedad Argentina de Inmunología.
Resumen:
Hepatitis B
virus (HBV) is the main infectious hazard for healthcare workers (HCW). The
HBV vaccine based on the recombinant surface virus antigen (HBs) protects a
high proportion of the vaccinees. All HCW should be immunized. Response
is evaluated by serum antibody (Ab) titers tested 30-60 days after the third
dose. A titer >10 mUI/mL is considered protective.
HCW have a much higher risk of getting HBV than the general population, so a
higher titer is often suggested for them (>100 mUI/mL). Many HCW were never
evaluated after immunization, so they are unaware of their vaccination status.
Countries differ in requiring or not booster shots.
Our objective was to study
the immune memory in vaccinees at different times after completion of a full course
of 3 HBV vaccinations.
Serum levels of anti-HBs Abs were evaluated by ELISA(anti-HBs, Radim, Italy) in 228 individuals. In
vitro cellular immune responses were evaluated using an HBsAg-specific proliferation assay with tritiated
thymidine in 11 samples so far. Participants work
at one of two institutions in Buenos Aires and signed an informed consent. This study was approved by the ethics committees of both centers.
Participants were classified by their Ab titers
in three groups: G1: <10 mIU / mL, G2: 10-100 mUI/mL and G3: >100
mUI/mL. Of all
cases 84.2% presented Abs>10 mIU / mL. In the literature 5-20%
non-responders (NR) are reported. Here 15.8% are in G1. They
must be reassessed after a secondcourse of vaccinations to be defined or
not as NR. Some HCW with previous high Ab titers showed decrease or lack
of them. To assess immunological memory despite low titer, proliferation
was tested. Proliferation was observed in those HCW initially in G3 (high
response), even with Ab levels dropped over time: (Previous/Present group) 6
G3/G3; 2 G3/G2; one G3/G1, while no proliferation was observed in two G1/G1 (no
response). These preliminary data suggest that cellular parameters could be
considered as markers of protection in HCW.