IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Clinical Case Presentation: Unusual complication in CLL-RANKL production in a CLL patient with bone lesions.
Autor/es:
IGNACIO LEDESMA; MERCEDES BORGE; GAMBERALE ROMINA; GIORDANO MIRTA; BEZARES FERNANDO RAIMUNDO; VICTORIA DELPINO
Lugar:
Punta del Este
Reunión:
Workshop; First IberoAmerican Meeting on Chronic Lymphocytic Leukemia (IbAMCLL-2013); 2013
Resumen:
Unusual Coplication in CLL: Clinical Case. RANKL production in CLL. We observed a CLL patient (JL) with bone lession associeated with CLL infiltration and high levels of RANKL in plasma. Osteoclast are the cells specialized in bone resorption in physiological conditions, but it has also been report an increase in ostocleast differentiation or in it?s activity in pathological condition associated with bone damage. This increase in osteoclastogenesis was produce by an overexpression of RANKL or others pro inflammatory conditions. Given that we found higher levels of RANKL in JL?s plasma compared to other CLL patients, our hypothesis was that this high concentration of RANKL was responsible for the bone lesions, presumably by inducing osteoclast differentiation. We first asked if the serum of JL patient was actually capable of inducing osteoclast differentiation. To test this we cultured monocytes derived from PB from health donors in the presence of the serum of the patient with bone lesions, or in the presence of other patients without the bone lesions, or in the presence of RANKL as a positive control. After 6 days of culture, ostoclasteogensis was determinated by the generation of this big, multinucleated, TRAP positive cells. TRAP is a phosphatase tippicaly expressed in osteoclast cells. Then we performed an Osteoclastogeneis assay, in with we evaluated the in vitro capacity of JL patient?s serum to induce the differentiation of monocytes into osteoclast. We found that: -Plasma from Patient JL has a high concentration of soluble RANKL which can induce osteoclast differentiation. -Osteoclast-like cells differentiated in the presence of plasma from patient JL were able to resorb dentin. -CLL cells from patient JL release soluble RANKL after in vitro stimulation.