IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Mechanisms involved in conjunctival immunological tolerance breakdown by eye drop preservatives
Autor/es:
JEREMÍAS GASTÓN GALLETTI; MARÍA LAURA GABELLONI; FLORENCIA SABBIONE; PABLO CHIARADÍA; MIRTA GIORDANO; JAVIER CASIRAGUI
Lugar:
Seattle
Reunión:
Congreso; ARVO Annual Meeting 2013; 2013
Institución organizadora:
Association for Research in Vision and Ophtalmology
Resumen:
ABSTRACT BODY: Purpose: To evaluate the molecular and celular mechanisms involved in the disruptive immune effect of benzalkonium chloride (BAK) on the ocular surface. Methods: A previously validated in vivo murine model was used (Mucosal Immunol. 2012 Jun 13. doi: 10.1038/mi.2012.44). In brief, Balb/c mice were given ovalbumin (OVA) alone or in combination with 0.01% BAK (B+O) daily for 5 days in both eyes, with and without nuclear factor κB (NFκB) pathway inhibitors: pyrrolidine dithiocarbamate (PDTC) and sulfasalazine (SSZ). On day 7, mice were immunized intraperitoneally with OVA in alum to evaluate the systemic response on day 14 as OVA-induced splenocyte proliferation by thymidine incorporation. To model in vitro the ocular surface epithelium, Pam212 cells were exposed for 15 min to different BAK concentrations (0.00001%-0.01%), then cultured for 96 h with syngeneic splenocytes and finally cell viability and expression of major histocompatibility complex class II (MHC-II) as activation marker were assayed. Results: In the in vivo model, OVA instillation induced conjunctival tolerance (43±9% of control systemic response, p