IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
H37RV MODULATES THE EXPRESSION OF DC-SIGN AND CD11B ON A549 CELL LINE.
Autor/es:
EVANGELINA A. LABORDE; LUCIANA BALBOA; SCHIERLOH, PABLO; LOPEZ, BEATRIZ; BARRERA, LUCÍA; RITACCO, VIVIANA; DE LA BARRERA, SILVIA; SASIAIN, MARÍA C
Lugar:
Lima
Reunión:
Congreso; Inmuno Perú 2012; 2012
Institución organizadora:
Asociación latinoamericana de Inmunología
Resumen:
The respiratory epithelium contributes to immune response against Mycobacterium tuberculosis (Mtb) playing a critical role in its dissemination. The aim of this study was to evaluate the Mtb effect on entry receptors and on ICAM-1 molecules, in 24h stimulated human alveolar A549 cell line with H37Rv and multi-drug resistant clinical isolates from Argentina: M strain (Haarlem) and Ra strain (LAM) by FACS. Our results are the first evidence for DC-SIGN and CD11b expression in A549. Furthermore, H37Rv up-regulated DC-SIGN expression (MIF y SE: Control: 183,7 + 12,64; H37Rv: 249,2 + 40,62) and CD11b expression (MIF + SE: Control: 161,5 + 25,77; H37Rv: 206,0 + 34,97 n=4); however, it was unable to modulate Mannose Receptor (MR) expression. In contrast, clinical isolates did not affect the basal expression of studied markers. Interestingly, neither H37Rv nor clinical isolates modulated the basal ICAM-1 expression. Additionally, Mtb was able to inhibit IFNg-mediated ICAM-1 induction on A549 cells. These results suggest that the Mtb genotype could influence the immune response of alveolar epithelium as well as its development during earliest stages of infection. These results highlight the role of alveolar epithelium in the immune response against Mtb infection.