C5A ANAPHYLATOXIN RECEPTORS MODULATE CYTOKINE PRODUCTION IN MACROPHAGE IMMUNE RESPONSE INDUCED BY MDR MTB CLINICAL ISOLATES

SABIO Y GARCÍA, CARMEN; YOKOBORI, NOEMÍ; BASILE, JUAN ; GONZÁLEZ, ALEJANDRA; ASQUINEYER, YANINA ; LÓPEZ, BEATRIZ; DE LA BARRERA, SILVIA; SASIAIN MCARMEN

Lima

Congreso; Inmuno Perú 2012; 2012

Asociación latinoamericana de Inmunología

Clinical isolates M and Ra are the most prevalent multidrug resistant strains of Mycobacterium tuberculosis (Mtb) in Argentina. These strains induce an altered Th1/Th2/Th17 profile in T cells. Growing evidences focus on the role of complement C5a anaphylatoxin receptors, C5aR and C5L2, in the modulation of cytokine (CK) production. However, little is known about its role in immunity against Mtb. On this regard, mice deficient in C5 component are more susceptible to tuberculosis (TB) being macrophages (Mac) more permissive to Mtb growth. In this study, we analyzed C5aR and C5L2 anaphylatoxin receptors modulation of Mtb-induced CK production on TB patients (TB) and healthy donor’s (HD) macrophages (Mac). Methods: Mac were isolated by adherence in plate and stimulated with H37Rv, M and Ra MDR strains for 18 hrs and IL-12 p40, IL-10 and TNF-a were measured in supernatants by ELISA. Results: On TB-Mac C5aR and C5L2 blockage increased H37Rv-induced IL-12p40 production and only C5aR blockage enhanced H37Rv- and Ra-induced IL-10 as well as Ra-induced TNF-a production . On HD, both receptors blockage tend to increased induced H37Rv- IL-12p40 production. Conclusion: These results show that C5a receptors differentially modulate cytokines production in Mac being these differences also dependent on the strains.