IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Activation of B Lymphocytes in the gut associated lymphoid tissue of weaned mice after intragastric inoculation of shiga toxin-producing Escherichia coli
Autor/es:
FERNANDEZ-BRANDO, RJ; CABRERA, G; BASCHKIER, A; MILIWEBSKY, E; MEJIAS, MP; PANEK, CA; RIVAS, M; PALERMO, MS
Lugar:
Paris
Reunión:
Congreso; 15th International Congress of Mucosal Immunology (ICMI 2011); 2011
Institución organizadora:
Society for Mucosal Immunology (SMI)
Resumen:
After mice intragastric inoculation of E.coli O157:H7 Stx+ strains (125/99), we observed an early decrease of B lymphocytes (BLym) percentage (%) in Peyer?s patches (PP), with a simultaneous increase in mesenteric lymph nodes (MLN) compared to controls: mice inoculated with a E.coli O157:H7 Stx- strain (605/03) or PBS (Ctrl). We aim to further study the activation and trafficking. Flow cytometry analysis showed evidence of BLym activation in 125/99- and 605/03-inoculated mice: at 12 h post-inoculation as an increased CD69+ BLym % in PP(mean±SD,n) (Ctrl=31.3±2.3,3; 605/03=43.1±4.5,3; 125/99=50.3±10.8,3; p