IDENTIFICATION OF GLYCOPROTEIN 1B (GP1B), LRP1 AND ANNEXIN II IN TUMOR CELL LINES

POWAZNIAK Y; KEMPFER AC; CALDERAZZO JC; ALONSO DF; LAZZARI MA; SÁNCHEZ LUCEROS A

KYOTO

Congreso; XXIII CONGRESS OF INTERNATIONAL SOCIETY ON THROMBOSIS AND HAEMOSTASIS; 2011

INTERNATIONAL SOCIETY ON THROMBOSIS AND HAEMOSTASIS

The association of cancer and thrombosis is common in patients with malignancies and has been shown that tumor cells, through receptors, interact with some hemostasis components. We studied the interactions which involve glycoprotein Ib (GPIb), a platelet receptor that binds to von Willebrand factor (VWF), low density lipoprotein receptor-related protein 1 (LRP1) that participates in the uptake and transport of FVIII to intracellular degradation pathways, and a fibrinolytic receptor, annexin II (AII) that function as a cofactor in the formation of plasmin binding plasminogen and tPA. Objective: To identify the presence of these receptors on tumor cells with different origin and metastatic capacity. Materials and Methods: Human tumor cell lines: MCF-7, MDA-MB-231, SK-MEL-28. Mouse tumor cell lines: B16-F0, F3II. Extraction and purification of total RNA using TRIzol. RT-PCR. Gel electrophoresis of PCR products. Sequence confirmation of PCR products (ABI PRISM 310). Results: (Table 1). mRNA GP1B     mRNA LRP1      mRNA AII MCF-7                          X *                     X*                 X** MDA-MB231               X                         X                   X SK-MEL-28                  -                          X                   X B16-F0                         X                         X                   X** F3II                              X                         X                   X X: presence confirmed by RT-PCR and sequencing, -: absence confirmed by RT-PCR and sequencing, *: reported positive, **: reported negative.  Conclusion: In this work we showed that most tumor cell lines studied had mRNA for GPIb, LRP1 and AII. SK-MEL-28 revealed no mRNA for GPIb. We do not agree with the absence of AII receptor mRNA described by other authors, considering as we could detect it. The description of these receptors in tumor cell lines will allow to assess the mechanisms and factors involved in the underlying prothrombotic state of cancer as well as to study its relationship with tumor and metastasis biology.