IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Intrahepatic and peripheral blood phenotypes of natural killer and T cells: differential surface expression of killer cell immunoglobulin-like receptors
Autor/es:
BELÉN, SANTIAGO; MONTAL, SILVINA; PODESTA, LUIS G.; MACHICOTE, ANDRÉS; IMVENTARZA, OSCAR; GALDAME, OMAR; MACHICOTE, ANDRÉS; IMVENTARZA, OSCAR; GALDAME, OMAR; PODHORZER, ARIEL; LAUFERMAN, LEANDRO; PODHORZER, ARIEL; MARCIANO, SEBASTIÁN; LAUFERMAN, LEANDRO; FAINBOIM, LEONARDO; MARCIANO, SEBASTIÁN; FAINBOIM, LEONARDO; BELÉN, SANTIAGO; MONTAL, SILVINA; PODESTA, LUIS G.
Revista:
IMMUNOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2018 vol. 154 p. 261 - 273
ISSN:
0019-2805
Resumen:
Deep characterization of the frequencies, phenotypes and functionalities of liver and peripheral blood natural killer (NK), natural killer T (NKT) and T cells from healthy individuals is an essential step to further interpret changes in liver diseases. These data indicate that CCR7, a chemokine essential for cell migration through lymphoid organs, is almost absent in liver NK and T cells. CD56bright NK cells, which represent half of liver NK cells, showed lower expression of the inhibitory molecule NKG2A and an increased frequency of the activation marker NKp44. By contrast, a decrease of CD16 expression with a potential decreased capacity to perform antibody-dependent cellular cytotoxicity was the main difference between liver and peripheral blood CD56dim NK cells. Liver T cells with an effector memory or terminally differentiated phenotype showed an increased frequency of MAIT cells,T-cell receptor-γδ (TCR-γδ) T cells and TCR-αβ CD8+ cells, with few naive T cells. Most liver NK and T cells expressed the homing markers CD161 and CD244. Liver T cells revealed a unique expression pattern of killer cell immunoglobulin-like receptors (KIR) receptors, with increased degranulation ability and higher secretion of interferon-γ. Hence, the liver possesses a large amount of memory and terminally differentiated CD8+ cells with a unique expression pattern of KIR activating receptors that have a potent functional capacity as well as a reduced amount of CCR7, which are unable to migrate to regional lymph nodes. These results are consistent with previous studies showing that liver T (and also NK) cells likely remain and die in the liver.