IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Massive plasmablast response elicited in the acute phase of hantavirus pulmonary syndrome
Autor/es:
MARINA GARCIA; CARLA BELLOMO; LUCIANA BALBOA; VALERIA P. MARTıNEZ; VERONICA I. LANDONI; MARIA TERESA CORDOBA; MARIA DEL CARMEN SASIAIN; AYELEN IGLESIAS; AGOSTINA BRUNO; GABRIELA C. FERNANDEZ,; PABLO SCHIELOH
Revista:
IMMUNOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2017 vol. 151 p. 122 - 135
ISSN:
0019-2805
Resumen:
Beside its key diagnostic value, the humoral immune response is thought to play a protective role in hantavirus pulmonary syndrome. However, little is known about the cell source of these antibodies during ongoing human infection. Herein we characterized B-cell subsets circulating in Andes-virus-infected patients. A notable potent plasmablast (PB) response that increased 100-fold over the baseline levels was observed around 1 week after the onset of symptoms. These PB present a CD3neg CD19low CD20neg CD38hi CD27hi CD138+/- IgA+/- surface phenotype together with the presence of cytoplasmic functional immunoglobulins. They are large lymphocytes (lymphoblasts) morphologically coincident with the ´immunoblast-like´ cells that have been previously described during blood cytology examinations of hantavirus-infected patients. Immunoreactivity analysis of white blood cell lysates suggests that some circulating PB are virus-specific but we also observed a significant increase of reactivity against virus-unrelated antigens, which suggests a possible bystander effect by polyclonal B-cell activation. The presence of this large and transient PB response raises the question as to whether these cells might have a protective or pathological role during the ongoing hantavirus pulmonary syndrome and suggest their practical application as a diagnostic/prognostic biomarker.