Nucleosomes and neutrophil extracellular traps in septic and burn patients

KAUFMAN, TOMÁS; MAGOSEVICH, DÉBORA; MORENO, MARÍA CAROLINA; GUZMAN, MARÍA ALEJANDRA; D'ATRI, LINA PAOLA; CARESTIA, AGOSTINA; FANDIÑO, MARÍA EUGENIA; FONDEVILA, CARLOS; SCHATTNER, MIRTA; KAUFMAN, TOMÁS; MAGOSEVICH, DÉBORA; MORENO, MARÍA CAROLINA; GUZMAN, MARÍA ALEJANDRA; D'ATRI, LINA PAOLA; CARESTIA, AGOSTINA; FANDIÑO, MARÍA EUGENIA; FONDEVILA, CARLOS; SCHATTNER, MIRTA

CLINICAL IMMUNOLOGY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Año: 2017 vol. 183 p. 254 - 262

1521-6616

NETosis is a host defense mechanism associated with inflammation and tissue damage. Experimental models show that platelets and von Willebrand factor (VWF) are key elements for intravascular NETosis. We determined NETosis in septic and burn patients at 1 and 4 days post-admission (dpa). Nucleosomes were elevated in patients. In septics, they correlated with Human Neutrophil Elastase (HNE)-DNA complexes and SOFA score at 1 dpa, and were associated with mortality. Patient´s neutrophils had spontaneous NETosis and were unresponsive to stimulation. Although platelet P-selectin and TNF-α were increased in both groups, higher platelet TLR-4 expression, VWF levels and IL-6 were found in septics at 1 dpa. Neither platelet activation markers nor cytokines correlated with nucleosomes or HNE-DNA. Nucleosomes could be indicators of organ damage and predictors of mortality in septic but not in burn patients. Platelet activation, VWF and cytokines do not appear to be key mediators of NETosis in these patient groups.