IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Monocyte-derived dendritic cells early exposed to Mycobacterium tuberculosis induce an enhanced T helper 17 response and transfer mycobacterial antigens
Autor/es:
BALBOA LUCIANA; GARCÍA MARINA; KVIATCOVSKY DENISE; BALBOA LUCIANA; DE LA BARRERA SILVIA; GARCÍA MARINA; KVIATCOVSKY DENISE; DE LA BARRERA SILVIA; SCHIERLOH PABLO; SASIAIN MARÍA DEL CARMEN; SCHIERLOH PABLO; SASIAIN MARÍA DEL CARMEN
Revista:
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY (PRINT)
Editorial:
ELSEVIER GMBH
Referencias:
Año: 2016
ISSN:
1438-4221
Resumen:
Tuberculosis (TB) is a complex disease, and the success of the bacterium depends on its ability to evade the immune response. Previously, we determined that Mycobacterium tuberculosis (Mtb) impairs the function of dendritic cells (DC), promoting the generation of cells that are poor stimulators of mycobacterial antigen-specific CD4T cells, which are required to control this persistent infection. In this study, we aimed to determine the mechanisms by which monocyte-derived DCs differentiated in the presence of Mtb (MtbDC) may impact on the proliferation of specific anti-mycobacterial T cells. We found that the presence of Mtb during monocyte-derived DC differentiation favours T helper (Th) 2 and Th17 polarization, in detriment of a Th1 response, compared to DC mature with Mtb. The bias on T cell polarization was associated to the profile of C-type lectin receptors expression found in MtbDC (DC-SIGNlow/MRlow/Dectin-1high). Alternatively, MtbDC release Mtb antigens (Ag) that can be taken up and presented by bystander DC, promoting the proliferation of CD4T cells, but to a lesser extent than direct presentation by Mtb-matured DC. In summary, we have further characterized the generation of MtbDC as an effective evasion strategy driven by the pathogen, leading to the inhibition of Ag-presentation and bias of T cell polarization towards Th2 and Th17 profiles, features which partially explain the persistence of Mtb in the host.