Glutathione S-transferase gene polymorphisms in celiac disease and their correlation with genomic instability phenotype

FUNDIA AF; WEICH N; CRIVELLI A; LA MOTTA G; LARRIPA IB; SLAVUTSKY I

Clin Res Hepatol Gastroenterol

Elsevier

Lugar: Philadelphia PA; Año: 2014 vol. 38 p. 379 - 384

2210-7401

Background and objective: Genomic instability and reduced glutathione S-transferase (GST)activity have been identified as potential risk factors for malignant complications in celiacdisease (CD). In this study, we assessed the possible influence of GST polymorphisms on genomeinstability phenotypes in a genetically characterised group of celiac patients from previousstudies.Methods: The deletion polymorphisms in GSTM1 and GSTT1 genes and the single-nucleotidepolymorphism GSTP1 c.313A>G were genotyped using PCR in a set of 20 untreated adult patientswith a known genomic instability phenotype and 69 age- and sex-matched healthy individuals.Results: The frequencies of variant genotypes in patients were GSTM1-null (30%), GSTT1-null(5%), GSTP1-AG (60%) and GSTP1-GG (15%), and they showed no differences from controls. Nosignificant differences were found in the genotype distribution based on telomere length. Caseswith GSTM1-null genotype (83%) and microsatellite stability were more frequent than those withgenomic instability. Moreover, carriers of GSTP1-variant genotype (73%) and stable phenotypewere significantly increased compared to unstable patients (27%) (P = 0.031). No differenceswere found according to the clinical-pathological characteristics of celiac cases.Conclusions: No association between GST polymorphic variants and celiac-associated genomicinstability was proven in our cohort. Future studies should explore the usefulness of otherbiomarkers to distinguish celiac patients who are susceptible to cancer development.