IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Differential expression of immunogenic proteins on virulent Mycobacterium tuberculosis clinical isolates
Autor/es:
SCHIERLOH P; KLEPP L; VAZQUEZ C; ROCHA R; BLANCO F; BALBOA L; LÓPEZ B; RITACCO V; BIGI F; SASIAIN MC
Revista:
BioMed Research International
Editorial:
Hindawi Publishing Corporation
Referencias:
Lugar: El Cairo; Año: 2014 vol. 2014 p. 1 - 13
ISSN:
2314-6133
Resumen:
Molecular epidemiology has revealed that Mycobacterium tuberculosis (Mtb), formerly regarded as highly conserved specie, displays a considerable degree of genetic variability that can influence the outcome of the disease as well as the innate and adaptive immune response. Recent studies have demonstrated that Mtb families found worldwide today differ in pathology, transmissibility, virulence and development of immune response. By proteomic approaches seven proteins that were differentially expressed between a local clinical isolate from Latin-American-Mediterranean (LAM)and from Haarlem (H) lineages were identified. In order to analyze the immunogenic ability, recombinant Rv2241, Rv0009, Rv0407 and Rv2624c proteins were produced for testing specific antibody responses. We found that these proteins induced humoral immune responses in patients with drug-sensitive and drug-resistant tuberculosis with substantial cross-reactivity among the four proteins. Moreover, such reactivity was also correlated with anti-Mtb-cell surface IgM, but not with anti-ManLAM, anti-PPD or anti-Mtb-surface IgG antibodies. Therefore, the present results describe new Mtb antigens with potential application as biomarkers of TB.