IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Neutrophils suppress gamma, delta T-cell function
Autor/es:
SABBIONE F; GABELLONI ML; ERNST G; GORI MS; SALAMONE G; OLEASTRO M; TREVANI A; GEFFNER J; JANCIC CC
Revista:
Eur J Immunol
Editorial:
WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Referencias:
Año: 2014 vol. 44 p. 819 - 830
ISSN:
1521-4141
Resumen:
Gamma delta T cells have been shown to stimulate the recruitment and activation of neutrophils through the release of a range of cytokines and chemokines. Here, we investigated the reverse relationship, showing that human neutrophils suppress the function of human blood gamma delta T cells. We show that the upregulation of CD25 and CD69 expression, the production of IFN-γ, and the proliferation of γδ T cells induced by (E)-1-hydroxy-2-methylbut- 2-enyl 4-diphosphate are inhibited by neutrophils. Spontaneous activation of gamma delta T cellsin culture is also suppressed by neutrophils.We show that inhibitors of prostaglandin E2and arginase I do not exert any effect, although, in contrast, catalase prevents the suppression of gamma delta T cells induced by neutrophils, suggesting the participation of neutrophil derived ROS. We also show that the ROS-generating system xanthine/xanthine oxidase suppresses γδ T cells in a similar fashion to neutrophils, while neutrophils from chronic granulomatous disease patients only weakly inhibit gamma delta T cells. Our results reveal a bi-directional cross-talk between gamma delta T cells and neutrophils: while gamma delta T cells promote the recruitment and the activation of neutrophils to fight invading pathogens, neutrophils in turn suppress the activation of gamma delta T cells to contribute to the resolution of inflammation.