IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Structural alterations in chronic lymphocytic leukemia. Cytogenetic and FISH analysis
Autor/es:
ANA TRAVELLA; LORENA RIPOLLES; ANNA AVENTIN; ANDREA RODRIGUEZ; RAIMUNDO BEZARES; MARIA ROSA CABALLIN ; IRMA SLAVUTSKY
Revista:
HEMATOLOGICAL ONCOLOGY
Editorial:
JOHN WILEY & SONS LTD
Referencias:
Año: 2013 vol. 31 p. 339 - 347
ISSN:
0278-0232
Resumen:
In this study, we described cytogenetics and FISH (fluorescence in situ hybridization) analysis performed in chronic lymphocytic patients (CLL) patients with structural alterations. Results were correlated with clinical characteristics. A total of 38 CLL patients: 16 cases with complex and 22 with simple karyotypes were studied. For comparison of clinical parameters, a control group of 78 CLL patients with normal karyotype and without FISH genomic alterations were also evaluated. We found 38 structural abnormalities not previously described in the literature, 28 (74%) of them were translocations. In cases with complex karyotypes, chromosomes 6, 8 and 13 were the most frequently involved in new alterations (9 each), followed by chromosomes 12, 14 and 15 (6 each). Chromosome 8p was particularly involved in losses, being 8p21-pter the commonest region of overlap. Cases with simple karyotypes, showed del(6q) as the most frequent alteration (39%). Del(9)(q11) was recurrent in our series. Analysis of clinical parameters showed significant differences in white blood count (p=0.005) and platelet count (p=0.015) between patients with structural alterations and the control group. In addition, patients with structural alterations had a significantly shorter time to first treatment (TFT) (29 months) than the control group (69 months) (p=0.037). Cases with complex karyotypes had a lower proportion of patients in Rai 0 clinical stage (15.4% vs 75%) (p=0.005) and higher â2 microglobulin levels (3.3µg/ml vs 2.5µg/ml) (p=0.037) than those with simple karyotypes. Furthermore, a shorter TFT (13 months) and overall survival (56 months) in the complex karyotypes group compared to controls (69 and 144 months, respectively) (p=0.015 and p=0.005, respectively), were also found. Our results support the importance of cytogenetic analysis for clinical outcome in CLL and suggest that the diversity of genomic alterations is much greater than previously appreciated.