- Hippocampal early changes in a minimal hepatic encephalopathy rat model.

PERAZZO, JC, TALLIS, S, CALTANA, L, ARONNE, MP, DELANTE, A, SOUTO, P, REINÉS, A, BRUSCO, A.

Val-David

Congreso; 14th Symposium of the International Society on Hepatic Encephalopathy and Nitrogen,; 2010

Graded portal-vein stenosis was used to induce rat minimal hepatic encephalopathy (MHE) whose main features are portal hypertension, moderate hyperammonemia and hippocampal mitochondrial alterations. These features were well established by day 14 post stenosis. We present a new view that shows the features on the 10th day after the stenosis in the rat hippocampus. Glial fibrillar acidic protein (GFAP) specific for astrocytes (A), S100~ protein localized in the cytoplasm of A, Nestine (N) localized in A, perycites and endothelial cells (ECs), microtubule-associated protein 2 (MAP-2) and neurofilament 200kDa, dendritic markers, Lycopersicum esculentum (tomato lectin) for microglia, and a euronal specific marker, were evaluated by immunohistochemistry. Beside this, Tripheniyltetrazolium (TTC) as a dye for hypoxic areas in rat's brain and Hypoxia Inducible Factor I a (HIF-l a) was evaluated by westem blot technique and by immunohistochemistry. At day lOA,