THE CAMP CONTENT IN WHOLE URINE AND EXTRACELLULAR VESICLES (EVS) AS POSSIBLE MARKERS OF ADPKD PROGRESSION. A PRELIMINARY REPORT

TOLEDO J; YANEFF A; ODDO E; DIANEZ R; FRAGA AR; SOSA MH; AZURMENDI P; ROSEMBERG ML; SOLERNO M; DAVIO CA; PERONI RN

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2019

Sociedad Argentina de Investigación Clínica

ADPKD is characterized by an intracellular calcium depletion that belongs to deficient activity of the polycystin 1 or 2 complex which, in turn, stimulates cAMP production by adenylate cyclases and inhibit its degradation by phosphodiesterases. However, the behavior of the cAMP in the disease progression is not completely elucidated. We had previously shown that urine in ADPKD is enriched on cAMP when compared to controls. On the other hand, EVs secreted in urine (namely urinary exosomes) play an essential role in cell-to-cell communication and are an emerging tool as renal disease progression biomarkers. Urine exosomes appears to be an accessible tool to measure intracellular parameters such as cAMP. Our objective was to evaluate the levels of urinary cAMP over time in ADPKD at a three-year follow-up. In addition, we analyzed whether urinary exosomes contain cAMP in ADPKD as well as in healthy volunteers.Urinary cAMP was measured in a 3-years follow-up of 15 ADPKD (36 ± 1 years, 7 women) and the data were compared with total kidney volume (TKV), GFR and osmolality. Urinary EVs were obtained from 4 ADPKD patients and 4 healthy controls with precipitation method. Using the radioligand binding assay, we measured the cAMP. The results show that the urinary cAMP varies timely in ADPKD by age (r = 0.72 p < 0.0001), together with known progression markers (VRT, GRF). Additionally, cAMP seems to be related to osmolal excretion (r=-0.4340, p