Dual modulation of glucocorticoid receptor activity by histamine H2 receptor signaling. Involvement of Rap, ERK and cAMP

KELLY A; DAVIO C; FERNANDEZ NC; ZAPPIA CD; TORRALBA V; SHAYO C; MONCZOR F

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2019

There are reports describing the interaction between membrane G-protein coupled receptor signaling and glucocorticoid receptor (GR) transcriptional activity. We have already reported that the signaling of the Gαs-coupled histamine H2 receptor (H2r) increased GR transcriptional activity. The aim of the present work was to study the molecular mechanisms of this effect. HEK293 cells were transfected with plasmids coding to H2r, GR and a GR-driven reporter gene TAT3-Luc. While pretreatment with 10 μM H2r agonist (amthamine), that augments cAMP levels, increased dexamethasone (dex)-induced GR activity in a 50% (p