ININFA   02677
INSTITUTO DE INVESTIGACIONES FARMACOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Sex differences in the modulation by baclofen of anxiety-like behaviour associated to nicotine withdrawal syndrome
Autor/es:
CALVO M; VARANI A; INDUNI A; BALERIO G
Lugar:
Rosario, Argentina
Reunión:
Congreso; Asociación Argentina de Farmacología Experimental. XLI Reunión Anual de Comunicaciones Científicas; 2009
Institución organizadora:
Sociedad Argentina de Farmacología Experimental
Resumen:
  SEX DIFFERENCES IN THE MODULATION BY BACLOFEN OF ANXIETY-LIKE BEHAVIOUR ASSOCIATED TO NICOTINE WITHDRAWAL SYNDROME Calvo M 1, Varani A 1, Induni A 1,2 and Balerio G 1,2 1ININFA (CONICET) y 2Cát. de Farmacología (FFYB, UBA) Junín 956, 5°Piso.  Buenos Aires. E-mail: gbalerio@ffyb.uba.ar   Increased anxiety is one of the motivational aspects of NIC withdrawal. We have previously demonstrated that baclofen (BAC), GABAB receptor agonist, was able to decreased somatic expression of NIC and morphine withdrawal syndrome. The aims of the present study were: a) to analyze if the anxiogenic effect associated to NIC withdrawal is influenced by sex, b) to evaluate the effect of BAC on the anxiety-like behaviour, in NIC abstinent mice of either sex. Swiss Webster albino mice received NIC (2.5 mg/kg; sc) 4 times daily, for 7 consecutive days. On day 8th, dependent mice received the nicotinic receptor antagonist mecamylamine (MEC; 2 mg/kg; sc) 1h after the last dose of NIC. A second group of dependent mice received BAC (2 mg/kg; ip) 45 min before MEC precipitated abstinence. Immediately the anxiety-like behavior was measured for 15 min in the elevated plus maze test. NIC withdrawal significantly decreased the percentage of time spent and the percentage of entries in the open arms (p<0.001) in male as well as in female mice. This effect was prevented by BAC pretreatment in male (p<0.001) but not in female mice. Our results demonstrate that: a) the anxiogenic response associated to NIC withdrawal is not influenced by sex, b) BAC prevented this anxiogenic effect associated to NIC withdrawal only in male mice. In conclusion, our results suggest a sexual dimorphism in the involvement of GABAergic system in the anxiety-like behaviour during NIC withdrawal. Supported by UBACYT  B016