ININFA   02677
INSTITUTO DE INVESTIGACIONES FARMACOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Comparation between acute and chronic postnatal stress in glutamatergic neurons in the rat brain
Autor/es:
MARIA MERCEDES ODEON; ADRIAN E SALATINO; MARIA LUZ ORTA; GABRIELA ACOSTA
Lugar:
Huerta Grande, Córdoba
Reunión:
Congreso; First Joint Meeting of the Argentine Society for Neurosciences (SAN) and the Argentine Workshop in Neurosciences (TAN); 2009
Institución organizadora:
SAN y Taller de Neurociencias
Resumen:
Comparation between acute and chronic postnatal stress on glutamatergic neurons in the rat Odeón María Mercedes, Salatino Adrián Emanuel, Orta María Luz, Acosta Gabriela Beatriz Instituto de Investigaciones Farmacológicas (ININFA) (CONICET-UBA). Junín 956. 5th floor, C1113AAD, Buenos Aires, Argentina E-mail: merodeon@hotmail.com It is well known that animals exposed to stressful stimuli during their early life develop different neurological disorders when they become adults. In this study, we evaluated the consequences of acute and chronic stress on adult brain on Glutamate transporter (GluT) in vitro, evaluating the uptake of [3H]-Glu by synaptosomes-enriched fractions isolated from rat cerebral frontal cortex (FC) and hippocampus (HIC) by time-course and kinetic studies. In acute stress, the rats were separated from their mothers and divided in two groups: control and stress. While control rats were moved to a separated cage, stress rats were exposed to cold stress (4ºC) during 1 h. In repeated stress the rats were separated from their mothers and exposed to cold stress (4ºC) for 1 h at postnatal day (PD) 7 during 20 days. These animals were allowed to a 30 days recovery period until adulthood. At the end of the stress period, animals were killed by decapitation. FC and HIC were dissected to study GluT and trunk blood samples were collected to determinate corticosterone levels. Acute stress results show an HIC increase in both affinity (Km) and maximum velocity (Vmax), while in the FC only the affinity was increased. Repeated chronic stress shows changes only in Vmax: rise in FC and decrease in HIC. The time-course on FC in acute stress shows an uptake decrease while in chronic stress the uptake was similar in both groups. HIC did not show significant differences either chronic or acute stress. The levels of corticosterone decreased in chronic stress. These results suggest that the exposure to early stressful adverse life events affects hypothalamic-pituitary-adrenal (HPA) axis and alter the glutamatergic neurons.