ININFA   02677
INSTITUTO DE INVESTIGACIONES FARMACOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ANTIHISTAMINES AND CORTICOIDS CO-TREATMENT RATIONALE. POTENTIATION OF GLUCOCORTICOIDS ANTI-INFLAMMATORY EFFECTS
Autor/es:
SOTO, ARIADNA; SHAYO, CARINA; MONCZOR FEDERICO; GRANJA GALEANO, GINA; GOLDMAN, ALEJANDRA; ZAPPIA, CARLOS DANIEL; FERNANDEZ, NATALIA; FITZSIMONS CARLOS
Lugar:
Mar del Plata
Reunión:
Congreso; LXI Reunión Anual de la SAIC; 2016
Institución organizadora:
SAIC
Resumen:
Antihistamines and glucocorticoids are used to treat many inflammatory conditions, such as allergic rhinitis and asthma, and in many cases they are co-administered. However, this association has no clear rationale and has arisen from clinical practice. We have previously reported the potentiating effects of histamine H1 receptor (H1R) signaling on glucocorticoid receptor (GR) activity in vitro. Here we evaluated the effect of the H1 antihistamine azelastine (Aze) on GR transcriptional activity induced by dexamethasone (Dex). HEK293 cells were co-transfected with plasmids coding for GR, H1R and a GR-responsive luciferase reporter plasmids to study transactivation (TAT3-Luc) or transrepression (IL6-Luc) of responsive genes. In both cases, preincubation with Aze 10 μM induced a three-fold increment in the response elicited by 0.1 nM Dex (p