ININFA   02677
INSTITUTO DE INVESTIGACIONES FARMACOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ROLE OF ADHESION MOLECULES IN THE SYNAPTIC REMODELLING INDUCED BY AN EXPERIMENTAL MODEL OF DEPRESSION AND THE ANTIDEPRESSANT FLUOXETINE
Autor/es:
PODESTÁ MF; LORENZO LÓPEZ, JR; SONNEMBERG, M; LÓPEZ, M; BRUSCO, A; WIKINSKI, S; REINÉS, A
Lugar:
Tandil, Buenos Aires
Reunión:
Congreso; XL Reucion anual de la Sociedad Argentina de Farmacologia Experimental; 2008
Institución organizadora:
Sociedad Argentina de Farmacologia Experimental
Resumen:
Hippocampal plasticity seems to be involved in the pathophysiology of depression and in the therapeutic actions of antidepressants. NCAM and N-cadherin adhesion molecules participate in building and maintaining synaptic structure but also play a role in neuroplasticity. The aim of this work was to examine the synapse morphology and adhesion molecule expression in the hippocampus of animals exposed to the learned helplessness (LH) paradigm. The effects of the antidepressant fluoxetine (FLX) were also studied. At the CA3 synapses of LH animals, synaptic cleft was wider and total number of vesicles was lower than controls, whereas length and thickness of postsynaptic density were, respectively, reduced and increased. While immunostainings for NCAM decreased and for PSA-NCAM, a less-adhesive form, increased in the CA3 region of LH rats, results were exactly the opposite in the CA1. In LH group, total NCAM and N-cadherin expression failed to differ from controls, possibly due to a counteracting effect between CA3 and CA1 contributions. Interestingly, FLX treatment strongly reduced total NCAM expression, accompanied by a slight N-cadherin diminution. Our results indicate that LH exposure induces a decrease in CA3 connectivity and that FLX might exert its action by reducing NCAM expression and so that inducing synaptic remodelling.