ININFA   02677
INSTITUTO DE INVESTIGACIONES FARMACOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
256.17/JJ12. Modafinil rescues methamphetamine-Induced cognitive deficits in object recognition memory in mice: Role of ERK1/2 phosphorylation in prefrontal cortex
Autor/es:
BETINA GONZALEZ; MARIANA RAINERI; NATALIA COLETTIS; JEAN LUD CADET; EDGAR GARCIA-RILL; FRANCISCO URBANO; VERONICA BISAGNO
Lugar:
San Diego, CA, USA.
Reunión:
Congreso; 2013 Society for Neuroscience Meeting.; 2013
Institución organizadora:
Society for Neuroscience
Resumen:
Chronic use of methamphetamine (METH) leads to long-lasting cognitive dysfunction in humans and animal models. Modafinil is a wake-promoting compound approved for the treatment of sleeping disorders and is being prescribed off label for the treatment of METH dependence. In the present study, we evaluated deficits in visual memory and ERK1/2 phosphorylation in the prefrontal cortex of mice treated chronically with METH; we also tested the ability of modafinil to rescue METH-induced cognitive and biochemical impairments in mice. After METH treatment (1mg/kg, sc, once daily for 7 days), mice performed the Novel Object Recognition (NOR) task, which evaluates visual memory and is sensitive to METH treatment. After the last METH injection, mice performed the NOR task which consisted of 3 habituation sessions to the open field (5 min a day, 3 consecutive days), training session (10 min, day 4), and a retention session (5 min, day 5). One hour before the training session, mice were given a single acute dose of modafinil (90 mg/kg, ip) or vehicle, and were exposed to two identical objects. After a 24 hr delay, mice performed the retention session where one of the objects was replaced by a new object. Sessions (time spent with objects and locomotor activity) were analyzed using an automated video tracking system (Ethovision XT7, Noldus). A preference index was calculated as the time spent exploring the novel object/total exploration time. Control and modafinil mice showed elevated preference indices compared to METH-treated mice (p