TOXIC DOSE OF ACETAMINOPHEN INDUCES SELECTIVE OLIGODENDROCYTE DEATH AND REACTIVE ASTROGLIA IN A PRIMARY MIXED GLIAL CELL CULTURE.

PERÉZ MJ; TALLIS S; NOVAK A; DELFANTE AE; RUBIO MC; GHANEM CI

San Diego

Congreso; Experimental Biology 2012; 2012

ASPET

Brain damage in acetaminophen (AP) overdose is associated with liver failure but its direct effects has not been evaluated. Aim: To study the effect of toxic and non-toxic doses of AP on astrocyte and oligodendrocyte precursor cell (OPC) viability in a primary mixed glial cell culture. MYM: Primary cultures of mixed glial cells were carried out according to McCarthy and de Vellis (1980). Cells were treated with vehicle (DMSO) or AP (1 and 20 mM). After 48 hs cell viability was assessed; astrocyte marker (GFAP) and OPC marker (PDGFR-alpha) expression was evaluated by western-blot (WB) and immunocytochemistry. Results: The 1 mM dose of AP did not produce significant differences in cell viability, while 20 mM exposure decreased in 40% cell viability (P