Analysis of limphocytic PGP activity

CORTADA C.M., ESCOBAR A., CURRAS V., BRAMUGLIA G., NISELMAN A.V., CARBALLO M.A., RUBIO M. C.

Córdoba- Argentina

Congreso; XXXVIII reunión anual de la Asociación Argentina de Farmaocología Experimenatal; 2006

Asocición Argentina de Farmacología Experimental

Analysis of limphocytic PGP  activity Cortada C.M., Escobar A., Curras V., Bramuglia G., Niselman A.V., Carballo M.A., Rubio M. C. Dept. de Farmacología y Matemáticas, Citogenética Humana y Genética Toxicológica (CIGETOX), Fac. de Farmacia y Bioquímica, UBA, Junín 956 PB (1113) Buenos Aires, Argentina. Email: eldecata@yahoo.com.ar   The aim of this work was to construct an histogram with a sample of 35 values of PGP and to study the performance of a binormal distribution adjustment. The sample size, in this initial stage was of 35 healthy individuals, men and women, between 20 and 60 years. PGP activity in lymphocytes (FR) was measured by the extrusion of Rodamina 123 determined by flow cytometry in the presence and absence of verapamil, a PGP inhibitor. We have obtained a normal distribution N (2,36; 0,52) when fitting a normal to the PGP histogram and we verified that this adjustment was overcome by the mixture of a proportion p=0,55 of a normal distribution with mean m1 = 1,98 and standard deviation s1=0,25 and another proportion (1-p)=0,45 of a normal distribution with mean m2 =2,83 and standard deviation s2=0,34. Two Goodness of fit tests were calculated, Chi-Square and Kolmogorov-Smirnov. For the first one there was obtained the statistic Ch-S-binormal=0.0007 (P=0,99 N.S); for the second K-S-binormal=0,09 (P=0,95 N.S). Therefore according to these tests, it is not rejected the supposition that the observed values come from the distribution F= 0,55 x N (1,98; 0,25) + 0,45 x N (2,83; 0,34).