ININFA   02677
INSTITUTO DE INVESTIGACIONES FARMACOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
INCREASES IN TRPV1 PROTEIN ABUNDANCE AND CGRP CONTENT IN ENDOTOXEMIC RATS
Autor/es:
ABRAMOFF T*, ORLIAC ML, PERONI RN, NEUMAN I#, PODESTÁ E# AND ADLER-GRASCHINSKY E
Lugar:
Córdoba, Argentina
Reunión:
Congreso; XXXVIII Reunión Anual de la Asociación Argentina de Farmacología Experimental; 2006
Institución organizadora:
Asociación Argentina de Farmacología Experimental
Resumen:
INCREASES IN TRPV1 PROTEIN ABUNDANCE AND CGRP CONTENT IN ENDOTOXEMIC RATS Abramoff T*, Orliac ML, Peroni RN, Neuman I#, Podestá E#  and Adler-Graschinsky E. ININFA (CONICET). # Departamento de Bioquímica, Facultad de Medicina (UBA), ARGENTINA. *tabramoff@ffyb.uba.ar The vasorelaxant effects of the endocannabinoid anandamide (AEA) are potentiated 6 hours after intraperitoneal administration of 5 mg/kg of lipopolysaccharide (LPS) in the rat vascular mesenteric bed (Orliac et al., J Pharmacol Exp Ther. 304:179-84, 2003). Since anandamide acts through the activation of TRPV1 vanilloid receptors and the consequent calcitonin gene-related peptide (CGRP) release, the aim of the present work was to study whether modifications in the TRPV1 expression as well as CGRP content and release could be linked to the enhanced anandamide-induced relaxations in endotoxemia. In tongues used as a representative systemic model of TRPV1 receptor expression, a band of 100 kDa equivalent to the estimated molecular weight of TRPV1 was increased 6 h after LPS administration. The CGRP-immunoreactivity in mesenteric beds was also increased 6 h after LPS. The overflow of CGRP increased in LPS-treated rats when mesenteries were perfused during 10 min with 10 µM anandamide. Moreover, relaxations to anandamide were potentiated by the protein kinase C activator 0.1 µM PMA in untreated mesenteries. The effect of PMA was accompanied by an increase in the overflow of CGRP induced by anandamide.  It is proposed that the overexpression of the TRPV1 receptors and the increased content of CGRP could contribute to the enhancement of anandamide effects during the endotoxemic shock. An eventual phosphorylation event linked to the overflow of CGRP could also participate in the enhanced relaxation caused by anandamide in endotoxemia. Supported by Grant BID 1728/OC-AR-PICT 5-14107.