Cross-talk between histamine H2 receptor and glucocorticoid receptor influences cell proliferation, differentiation and apoptosis in acute myeloid leukemia cells

SHAYO, CARINA; TORRALBA-AGU, VALERIA; ROCHA VIEGAS, LUCIANA; ZAPPIA, C. DANIEL; MONCZOR, FEDERICO

Mar del Plata

Congreso; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC).; 2023

Sociedad Argentina de Investigación Clínica (SAIC).

Numerous studies investigated the individual administration of histamine (HA) and glucocorticoids (GCs) for treating acute myeloid leukemia. However, the joint application of these drugs remains unexplored. Our previous findings revealed that HA and amthamine (Amtha), a histamine H2 receptor agonist, increases the transcriptional activity of the dexamethasone (Dex)-induced glucocorticoid receptor. In this study, we aimed to assess whether this cross-talk had any impact on cell proliferation, differentiation and apoptosis using the U937 leukemic cell line. 96-hour exposure of U937 cells to low doses of Dex (1-10 nM) led to an approximately 50% increase in cell proliferation, whereas higher doses (>1 μM) caused a 50% reduction in cell proliferation (p