ININFA   02677
INSTITUTO DE INVESTIGACIONES FARMACOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells
Revista:
MOLECULAR AND CELLULAR ENDOCRINOLOGY.
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Lugar: Amsterdam; Año: 2016 vol. 1 p. 259 - 267
ISSN:
0303-7207
Resumen:
Epidermal growth factor (EGF) has beensuggested to play a key role in the maintenance of epithelial cell survivalduring lactation. Previously, we demonstrated that EGF dependent activation ofPI3K pathway prevents apoptosis in confluent murine HC11 cells cultured underlow nutrient conditions. The EGF protective effect is associated with increased levels of theantiapoptotic protein Bcl-XL. Here, we identify the EGF-dependent mechanism involved in cellsurvival that converges in the regulation of bcl-X expression byactivated CREB. EGF induces Bcl-XL expression through activation of a unique bcl-Xpromoter, the P1; being not only the PI3K/AKT signaling pathway but alsothe increase in cAMP levels and the concomitant PKA/CREB activation necessaryfor both bcl-XL upregulation and apoptosis avoidance. Results presented in thiswork suggest the existence of a novel connection between the EGF receptor andthe adenylate cyclase that would have an impact in preventing apoptosis underlow nutrient conditions.