Exposure to Endocrine Disruptors Alters Inflammatory Markers In Mouse Hypothalamus In-Vivo and In-Vitro

CARLOS LIBERTUN; JUAN MANUEL RIAÑO GOMEZ; VICTORIA AR LUX-LANTOS; ELEONORA M. SORIANELLO; MARINA O. FERNANDEZ

Virtual

Congreso; SETAC Latin America 14th Biennial Meeting; 2021

SETAC Latin America

Bisphenol A (BPA) and Benzophenones (BPs) are endocrine disrupting chemicals (EDC). Previously, we showed that the in-vitro exposure to BPA, BP2 or BP3 decreased Kiss-induced GnRH gene expression in GT1-7 cells (Dr. Pamela Mellon, UCSD). In this study, we analyzed the in-vitro exposure of GT1-7 cells or isolated hypothalami from adult Balb/c males to BPA, BP2 or BP3 (Sigma, 1x10-9 M) on cytokine and glial fibrillary acidic protein (gfap) gene expression. After the incubations, RNA was extracted, reverse transcribed and qPCR performed using specific primers. The in-vivo exposure to BP2 or BP3 (250 µg/kg/day, orally, for five days) effects on gene expression in C57Bl/6 mice was also analyzed. Animals were sacrificed, brains rapidly dissected and placed in dry ice, and kept -80 ºC until processed. Micropunches that contained the Anteroventral Periventricular (AVPV) or Arcuate (ARC) nucleus were obtained and RNA was extracted, reverse transcribed and gene expression analyzed by qPCR. Results were expressed as Mean±SE and analyzed by ANOVA using Statistica (Statistica v StatSoft Inc, USA).Twenty-four hour BPA increased il18 expression in GT1-7 cells (C=1.0±0.04, BPA=1.2±0.1, T-test p