In-vitro effects of BPA, BP2 and BP3 on cell proliferation in a mature GnRH neuronal cell line, GT1-7 cells

LIBERTUN, CARLOS; SORIANELLO, ELEONORA; FERNANDEZ, MARINA OLGA; RIAÑO GÓMEZ, JUAN MANUEL; LUX-LANTOS, VICTORIA

VIRTUAL por la pandemia de COVID-19

Congreso; IV Reunión Conjunta de Sociedades de Biología de la República Argentina; 2020

Sociedades de Biología de Cuyo, Córdoba y Tucumán con la participación de la Sociedad Argentina de Biología, la Sociedad de Biología de Rosario y la Sociedad Chilena de Reproducción y Desarrollo

Previously we showed that the in-vitro exposure to BPA, BP2 y BP3, endocrine disruptors, and E2, (1x10-7 and 1x10-9 M, 24 hs) increased cell proliferation in an immature GnRH cell line, GN11 cells (Susan Wray, USA). The aim of this study us to evaluate the effects of the in-vitro exposure of the aforementioned compounds on cell proliferation in GT1-7 cells (mature GnRH neurons, Pamela Mellon, UCSD, USA). Cell proliferation was evaluated using a Non-Radioactive Cell Proliferation Assay, MTS (Promega, WI, USA), after BPA, E2, BP2 and BP3 exposure (1x10-7 and 1x10-9 M, 24 hs). We also evaluated if the estrogen receptor antagonist ICI 182780, (1x10-6 M) was able to block the effects. Results were recorded as Abs490/Abs490(Control), presented as Mean±SE and analyzed by Repeated measures ANOVA with a Fisher posttest (Statistica, StatSoft, OK, USA). Neither BPA nor E2 modified cell proliferation (ANOVA ns, n=5). BP2 did not modify cell proliferation either, but BP3 increased cell proliferation compared to control values [Control=1±0.03; BP2-7=0.92±0.12; BP2-9=0.94±0.11; BP3-7=1.29±0.13; BP3-9=1.29± 0.09; Repeated measures ANOVA p