Antiinflammatory actions of Tibolone in the spinal cord of experimental autoimmune encephalomyelitis mice

MANCINO DALILA; GARAY L.; GARCIA-SEGURA LM.; ALEJANDRO DE NICOLA

Congreso; Reunión anual de Sociedades Biociencias SAIC-SAI-SAFIS; 2020

Tibolone (TIB) is a synthetic estrogenic compound with tissue-specific actions commonly used to prevent symptoms in postmenopausal women. The tissue selectivity of TIB depends on its specific local transformation to active metabolites that can exert estrogenic, progestinic and androgenic activities. Recently, antioxidant and anti-inflammatory actions of TIB have been described in the CNS. In this study we assessed the effect of TIB on the clinical outcome, inflammation and myelination in the spinal cord of experimental autoimmune encephalomyelitis (EAE) mice model of multiple Sclerosis (MS). To this purpose, female C57BL/6 mice were divided into control (CTRL), EAE receiving TIB (0.08mg/kg s.c every other day from the day of induction (EAE-TIB) or EAE receiving vehicle (EAE-V) groups. Spinal cord tissues were collected at day 17 post induction and subjected to qPCR and Western Blot analysis. EAE-TIB group showed decreased microglial reaction revealed by lower CD11B and toll like receptor 4 (TLR4) mRNAs expression respect of EAE-V (p