IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effects of periconceptional etanol ingestión on different collagen types and metalloproteinases in murine implantation sites at midgestation.
Autor/es:
COLL TAMARA ANAHÍ; FONTANA VANINA; FAVARO RODOLFO R; CALVO JUAN CARLOS; TENORIO ZORN TELMA MARÍA; CEBRAL ELISA
Lugar:
Bourbon Convention Ibirapuera, San Pablo, Brasil.
Reunión:
Congreso; XV Meeting of the Brazilian Society for Cell Biology; 2010
Institución organizadora:
Brazilian Society for Cell Biology
Resumen:
Periconceptional alcohol ingestion causes organogenic embryo anomalies, growth restriction and increased early miscarriages. The matrix metalloproteinases MMP-9 digest the types IV and V collagens and MMP-2 also degrades types I and III, playing a role in remodeling of the extracellular matrix (ECM), decidualization, angiogenesis and vascularization, trophoblastic migration and invasion of decidua. To evaluate if periconceptional ethanol ingestion alters distribution, localization and expression of collagens types I, III, IV and V, and MMP-9 in vascular mesometrial decidua (VMD) and trophoblastic (T) tissues, adult CF-1 female mice were treated (TG) with 10% ethanol in drinking water for 17 days previous and during gestation up to day 10 of gestation, and compared to control group (CG). Implantation sites were processed for picrosirius staining for fibrillar collagens, and immunohistochemistry for I, III, IV, V collagens and MMP-9. While in VMD-CG was observed a defined and continuous picrosirius stain in basal membranes of vessel endothelium, the VMD-TG staining was undefined and discontinuous. Collagen III had similar immunostain in the VMD-CG and VMD-TG. The immunofluorescence pattern for IV and V collagens was increased in VMD-TG vs VMD-CG. A slight mark of MMP-9 was detected in VMD-CG and T-CG, while the immunoreactivity was significantly reduced in VMD-TG and T-TG. These results suggest that periconceptional alcohol ingestion leads to reduced MMP-9 immunostaining with diminished collagens IV and V digestion, and diminished picrosirius stain, thus indicating potential structural alterations in fibrillar collagens in VMD.