Correlation between LXRs expressions and lipid parameters in rats fed with a high fat diet

FERESIN, G. E.; REY, M.; COIRINI, H.; KRUSE, M. S.

Mar del Plata

Congreso; Reunión Anual de Biociencia 2019 - SAIC-SAFE-SAB-SAP-NANOMEDar-AACyTAL-HSC; 2019

Sociedad Argentina de Investigación Clínica

The liver X receptors (LXRs) are keyfactors in cholesterol metabolism and also participate in themetabolism of fatty acids, triglycerides (TG) and glucose, amongother functions. There are two subtypes, LXRa and LXRb, whichare equally functional. Previously, we described a link betweenincreased plasma TG and hypothalamic LXRs expressions inglucose-intolerant rats, revealing that these receptors areprobably sensitive to peripheral changes of lipids. In this work,adult male rats received normal chow diet (C) or high fat diet(HF; C + 38 % bovine fat + 2 % cholesterol + 0.2 % cholicacid). Total cholesterol (TC) and TG levels were measured inblood samples by using commercial kits; hypothalamic andhepatic LXRs expressions levels were determined by WB. Thesequantifications were performed every 2 weeks through an 8weeks period. The TC levels in HF group were higher than C at allthe weeks evaluated (42.80-93.70 %; p