FKBP59 enhances FK506 neuroprotection in the Drosophila wing axotomy paradigm

RAMOS HRYB ANA BELÉN; PAGANI, MARIO RAFAEL; GODOY, LUCIANA FLORENCIA

California

Conferencia; 9th Annual Laboratory Animal Science Virtual Conference; 2020

North American 3Rs Collaborative (NA3RsC), the UK?s National Centre for the 3Rs (NC3Rs) and New Jersey Association for Biomedical Research (NJABR)

Transection of spinal cord is commonly used to study the FK506 effects. This invasive intervention causes distress and has low reproducibility. We studied FK506 effects on Drosophila wing axotomy paradigm. FK506 treatment reduced the nerve damage and increased neuronal soma number in injured wings. fkbp59 overexpression enhanced the FK506 neuroprotection. The wing axotomy paradigm is suitable to elucidate the neuroprotective mechanisms of FK506.