COX 1 AND COX 2 INHIBITIONS BY A DECOCTION OF ANDEAN SPECIES FROM SAN JUAN PROVINCE, ARGENTINE

COIRINI, H.; TAPIA, A.; LIMA, B.; FERESIN, G.E.

San Luis

Congreso; XXXVII Reunion Cientifica Anual de la Sociedad de Biologia de Cuyo; 2019

Plants are a source of bioactive metabolites; the ability to act inmultiple signalling pathways characteristic of inflammatory processes has beenreported. The aim is to obtain alternative drugs with higher potency and lowerundesirable effects. The species Azorella cryptantha, Oxaliserythrorhiza, Pachylaena atriplicifolia and Tetraglochinameghinoi that grow in Andes scopes from San Juan, Argentine, are used totreat illness related to inflammatory processes in traditional medicine. Thedry aerial parts were used to prepare decoctions (DC) (10% w/v), and wereassayed to inhibit cyclooxygenase enzymes (COX-1 and COX-2). The effect wascalculated the percent inhibition of COX isoforms measured by colorimetricassay (Kit No. 701050, Cayman Chemical), diclofenac sodium and etoricoxib wereused as reference inhibitors (1-2 μg/ml). The DCs wereevaluated at 18, 36 and 72 μg/ml. Regarding COX-2,DC from A. cryptantha and O. erythrorhiza were actives withinhibitory values of 82.10 and 86.97% respectively, to a concentration of the72 μg/ml. On the other hand, all DCs were not selective to inhibit COX-2,the percentage of inhibition in COX-1 was lower. These results showed that A.cryptantha and O. erythrorhiza are consistent with the traditional useof these species in phytotherapic preparations with a potentialanti-inflammatory effect, by blocking the COX-2 proinflammatory enzyme pathway.PIO CONICET SECITI 022. CICITCA UNSJ.