MYELINOGENESIS IS ACCOMPANIED BY AN INCREASE IN THE MRNA OF NEUROSTEROIDOGENIC MACHINERY IN THE CEREBELLUM OF POSTNATAL MICE

LARA, AGUSTINA; LEICAJ, MARÍA L.; DE NICOLA, ALEJANDRO F.; LIMA, ANALIA; GARAY, LAURA I.

Mar del Plata

Congreso; SAIC. SAI. SAFIS 2018; 2018

Sociedad Argentina de Investigación Clínica

The novo synthesized steroids influence various important physiologic and neurotropic functions that occur in the brain during early postnatal development. Progesterone and its reduced metabolite allopregnanolone promote the dendritic growth, spinogenesis, and synaptogenesis of cerebellar Purkinje cells. In order to assess the influence of locally synthetized steroids on physiologic myelination, we studied the expression of the protein involved in the neurosteroidogenic rate-limiting step of cholesterol entry to the mitochondria, the steroid acute regulatory protein (StAR), the enzymes P450scc (cholesterol side-chain cleavage) and 5-alpha-reductase and myelin basic protein (MBP) mRNAs in the mouse cerebellum at postnatal day (P) 5, 18 and 35 by quantitative PCR. We found a pronounced increased in the expression of MBP mRNA at P18 and P35 compared to P5 (p