BONE MARROW-DERIVED MESENCHYMAL STRO- MAL CELLS INDUCE THE RELEASE OF FREE CIRCU- LATING MICRORNAS BY TRIPLE NEGATIVE BREAST CANCER CELLS.

BORZONE FRANCISCO RAÚL; SCHARGORODSKY MARCOS; CHASSEING NORMA ALEJANDRA; PICCIONI FLAVIA; RODRIGUEZ SOFIA; DALTON NICOLAS; DE SIERVI ADRIANA ; FARRE PAULA LUCIA ; ROTH FEDERICO; MASSILLO CINTIA

Mar Del Plata

Congreso; LXIII Reunión Anual Sociedad Argentina de Investigación Clínica (SAIC); 2018

SAIC

7KHPDLQFDXVHRIGHDWKLQSDWLHQWVZLWKEUHDVWFDQFHU%&LVGXHto the metastasis of the primary tumor. Particularly, 60-70% of paWLHQWVZLWKDGYDQFHG%&GHYHORSERQHPHWDVWDVHV,WKDVEHHQUHported that mesenchymal stromal cells (MSCs), that include mesenchymal stem cells, precursor and progenitor cells, stimulate breastcancer cells to induce proliferation, invasion and migration to otherniches, through several factors. MicroRNAs (miRNAs) are key reguODWRUVRIWXPRULJHQHVLVDQGWXPRUSURJUHVVLRQDQGLWLVQRZNQRZQWKDWWKH\ FDQ FLUFXODWHLQWKHSHULSKHUDOEORRGZKLFKPDNHVWKHPpromising biomarkers. Previous results from our group establishedDOLVWRIPL51$VREWDLQHGIURPEUHDVWDOORJUDIWVJHQHUDWHGE\7WULSOHQHJDWLYH FHOOV LQ PLFH ZKLFK DUH GLIIHUHQWLDOO\ H[SUHVVHG LQDGYDQFHG VWDJH YVHDUO\ VWDJH,QDGGLWLRQWKH\ZHUHDOVRIRXQGLQWKH SODVPD RIWKHVHPLFH,QWKLVZRUN RXU DLPZDVWR LGHQWLI\WKHPL51$VUHOHDVHGE\06&VDQG7WXPRUFHOOVLQWHUDFWLRQ:Hgenerated a conditioned medium (MCo) from a primary culture ofERQHPDUURZ06&V0&R06&VIURPPLFH7KHQ7FHOOVZHUHH[SRVHGRUQRWWR0&R06&V$IWHUK7FHOOVZHUHZDVKHGDQGLQFXEDWHGZLWKFXOWXUHPHGLXPIRUK0&R06&V77RWDO51$ZDVLVRODWHGIURPWKHVH0&R0L51$VUHOHDVHGWRWKHPHGLXPZHUHGHWHFWHGXVLQJVWHPORRS57T3&55HVXOWVVKRZHGWKDWMCo-MSCs exposure increased the release of miR-125b-5p, miR-SDQGPL5SE\7FHOOV)XUWKHUPRUHPL5SDQGPL5SZHUHUHOHDVHGE\06&V:HFRQFOXGHWKDW06&VLQGXFHWKHUHOHDVHRIPL51$VE\7FHOOVPDLQO\PL5SDQGPL5SZKLFKDUHNQRZQWREHLQYROYHGLQWKHSURFHVVHVRIPHVHQFK\-mal-epithelial transition, proliferation and migration of breast tumorFHOOVDVZHOODVLQWKHDFWLYLW\RIWXPRUDVVRFLDWHGÀEUREODVWV