?SPHINGOLIPID CERAMIDE-1-PHOSPHATE (C1P) IN OVARY AS A PROMISING STRATEGY FOR DELAYING OVARIAN AGING?

PASCUALI, NATALIA; VELAZQUEZ C1, NEGRI MONTES L; ABRAMOVICH DALHIA; LEOPODINA SCOTTI; HEBE A MENA; SOLEDAD NEGROTTO; OUBIÑA, GONZALO; DE ZUÑIGA IGNACIO; FERNANDA PARBORELL

Buenos Aires

Congreso; XX Jornadas Anuales de la Sociedad Argentina de Biología y XVII Jornadas de la Sociedad Uruguaya de Biociencias.; 2018

Sociedad Argentina de Biología (SAB)

As many women postpone childbearing over 38 years a large portion of aged female becomes infertile. Ovarian aging is dominated by the progressive loss of primordial follicles and a decline in oocyte quality. An active blood supply via ovarian angiogenesis seems to be essential for the induction of oocytes with good quality. Activation of ovarian angiogenesis has emerged as a new strategy to halt age-related decline of ovarian response. Endothelial progenitor cells (EPCs) are a population of bone marrow-derived mononuclear cells thought to participate in endothelial repair. Since the bioactive sphingolipid C1P is an important proangiogenic factor, our aim was to: 1) evaluated the local effect of C1P on EPCs mobilization and on ovarian angiogenesis in aged female mice and 2) evaluated the angiogenic potencial of follicular fluid (FF) from aged patients in the presence of C1P in endothelial cells (ECs). Aged female mice (26-31 weeks) received C1P (10µl/ovary; 50µM) under the bursa of the ovaries. The animals were sacrificed 2 and 7 days later, and the ovaries were isolated. Young mice (6-9 weeks) were used as control. Prior to surgery and sacrifice, peripheral blood (PB) was extracted from the maxillary cavity to evaluate by flow cytometry EPCs mobilization (VE-Cadherin+, Sca-1+). Histological and immunohistochemical analysis (VW: endothelial cell marker, α-SMA periendothelial cell marker), and Western blot (VEGF-A) were performed. ECs were incubated in the presence of FF from aged patients (38-42 years) and young patients (