GLYCOSYLATION SIGNATURE AND GALECTIN-1 REGULATED SUPPRESSIVE PROFILE OF MYELOID-DERIVED SUPPRESSOR CELLS IN HEALTH AND CANCER

BACH CAMILA; ADA G. BLIDNER; GABRIEL A RABINOVICH

Toscana

Congreso; Gordon Conference; 2019

Gordon

Myeloid Derived Suppressor Cells (MDSCs) are a heterogeneous population of immature myeloid cells that give rise to DCs and macrophages; in pathologic conditions, such as inflammation, infection and cancer these cells stop their differentiation programs and become suppressive. If this conditions resolve, standard myelopoiesis is restored. However, there may be a sustained myelopoiesis which leads to the accumulation of immature myeloid cells with suppressive activity. The glycosylation signature of a cell can bring important information about its selective interaction with endogenous glycan-binding proteins (e.g. Galectin-1) present in inflammatory or tumor microenvironments. Here we examined the glycosylation profile of MDSCs in normal conditions and cancer settings. First, we examined the glycosylation pattern of MDSCs during the differentiation process. We found that Ly6g+Ly6cint/lowCD11b+ and Ly6g-Ly6chighCD11b+ populations presented differential glycosylation profiles: Ly6g-Ly6chighCD11b+ cells showed more abundant non-sialylated core 1 O- glycans branching (p