In-vitro exposure to Bisphenol A increases GFAP gene expression in whole hypothalami and IL-18 gene expression in GT1-7 cells.

FERNANDEZ, MARINA O.; LIBERTUN CARLOS; RIAÑO-GOMEZ JUAN; MARIGLIANO CAMILA; LUX LANTOS V

Congreso; XX Jornadas anuales de la Sociedad Argentina de Biologia y la XVII Jornada de la Sociedad Uruguaya de Biociencias.; 2018

IN-VITRO EXPOSURE TO BISPHENOL A INCREASES GFAP GENE EXPRESSION IN WHOLE HYPOTHALAMI AND IL-18 GENE EXPRESSION IN GT1-7 CELLS Fernandez M1, Marigliano C1, Riaño-Gomez J1, Libertun C1,2, and Lux-Lantos V1. 1-Laboratorio de Neuroendocrinología-Instituto de Biología y Medicina Experimental (IByME-CONICET) and 2-Facultad de Medicina-UBA. Email: mfernandez@dna.uba.ar.Bisphenol A (BPA), a monomer of polycarbonate plastics, and Benzophenones (BPs), UV-filters, are endocrine disrupting chemicals (EDC) found in everyday products. In this study we analyzed the effects of the exposure to BPA, BP2 and BP3 on Glial fibrillary acidic protein (GFAP) and IL-18 (a pro-inflammatory cytokine expressed in GnRH neurons) in whole hypothalami isolated from adult Balb/c males and IL-18 in mature GnRH neurons (GT1-7 cells, donated by Dr. Pamela Mellon, UCSD). Hypothalami were incubated in Krebs-Ringer medium in the presence or absence of BPA, BP2 or BP3 (1x10-9M) or medium alone (C) for six hours. After the incubation, hypothalami were homogenized in Tri-Reagent (Molecular Research Center, OH, USA) and mRNA extracted for Gfap and Il-18 analysis by qPCR. GT1-7 cells were cultured in 12-well plates (200000 cells/well) in DMEM supplemented with 10% FBS, pyruvate and Penicillin/Streptomicin (complete medium). After 24 hours, media were changed to DMEM without phenol red, supplemented with 10% charcoal-stripped FBS, pyruvate and Penicillin/Streptomicin (stimulation medium). Cells were incubated with BPA, BP2, BP3 (1x10-9M) or vehicle (C) in stimulation media for 24 hours. RNA was extracted using Tri-Reagent and Il-18 analyzed by qPCR. RNA (1μg) was reverse transcribed and qPCR performed using specific primers. Hypothalami exposed to BPA showed increased Gfap gene expression (C=0.82±0.16, BPA=1.70±0.39; ANOVA p