A GABAB antagonist increases ovarian Cyp19a1 and Esr1/2 mRNA in PND6 mice and delays puberty onset.

BIZZOZZERO MARIANNE; LIBERTUN CARLOS; TABARES FLORENCIA; DI GIORGIO NOELIA P; LUX LANTOS, V.

Congreso; 9th International Congress of Neuroendocrinology; 2018

A GABAB antagonist increases ovarian Cyp19a1 and Esr1/2 mRNA in PND6 mice and delays puberty onsetBizzozzero Hiriart Marianne, Di Giorgio Noelia, Tabares Florencia, Libertun Carlos and Lux-Lantos Victoria. Instituto de Biología y Medicina Experimental (IBYME-CONICET, Buenos Aires, Argentina). GABAB antagonist (CGP55845) administration to neonatal BALB/C mice significantly decreased ARC Kiss1 expression in both sexes. Neither Cyp19A1, Esr1/2, Pgr hypothalamic expression were not affected by this treatment. Nevertheless, ovarian and testicular E2 contents were significantly increased in CGP-treated mice. Here we analyzed Cyp19A1, Esr1/2, Kiss1 and Kiss1r in the ovaries of control and CGP-treated neonates and determined body weight (BW), anogenital index (AGI=AGD/BW) and puberty onset.Female Balb/c mice were injected with CGP55845 (1 mg/kg, sc) or saline from postnatal day 2 (PND2) to PND6±1, three times/day (8AM, 1PM, 6PM). One set of mice was sacrificed at 3PM (after two injections on the last day). One ovary was collected to determine Cyp19A1, Esr1/2, Kiss1 and Kiss1r expression by qPCR; the other ovary was used to analyze ovarian morphology. In the second set of animals BW and AGI were evaluated on PND7, PND14 and PND21 and puberty onset was determined by vaginal opening (VO).CGP55845 increased Cyp19A1, Esr1 and Esr2 expression in neonatal ovaries (Cyp19A1: CTRL:0.97±0.32 vs CGP:2.5±0.55: p