Trypanosoma cruzi infection affects adipocyte function in vivo and in vitro

VILLAR S; D´ATTILIO L; PEREZ A; GONZALEZ F; PACCINI MF; PIWIEN PILIPUK G; TONEATTO J; BOTTASSO O

Buenos Aires

Congreso; Reunión conjunta de Sociedades de Biociencias y 65ta Reunión de la SAI; Buenos Aires, 13-17 Noviembre 2017; 2017

Reunión Conjunta de Sociedades de Biociencia

Adipose tissue (AT) influences energy homeostasis and inflammationand also acts as Trypanosoma cruzi (Tc) reservoir. Werecently showed that C57BL/6 mice infected with Tc displayed anexacerbated pro-inflammatory milieu associated with metabolic abnormalitieslike manifest hypoglycemia, hypoleptinemia and a markedlyloss of AT mass, which may play a role in lethality. Probably,pro-inflammatory factors released during infection may be involvedin lipolysis and also influence adipocyte function. In order to characterizeAT wasting, we evaluate in vivo how Tc infection affects AThistology, features of inflammatory infiltrate and the expression offactors involved in inflammation, lipolysis and adipogenesis. Otherseries of studies were carried out in vitro using 3T3-L1 adipocytesexposed to plasmas derived from healthy (Co) and Tc-infected mice.Results: After 17 days post-infection (17 dpi), adipocyte numberand size diminished [size(pixel2x105) media±SEM; Co=2.7±0.2;Tc=1.3±0.1, p