Involvement of Wnt/B-catenin inhibition in ovarian cancer cell lines proliferation and apoptosis

GRISELDA IRUSTA; TESONE, MARTA; BOCCHICCHIO, SEBASTIAN

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Wnt/β-catenin is a highly conserved pathway which regulates proliferation, apoptosis and differentiation. While it has been demonstrated to participate in cell growth of different biological systems, no studies have been performed in ovarian cancer cell growth regulation. To evaluate the involvement of Wnt/β-catenin pathway in ovarian cancer cell proliferation and apoptosis, two human epithelial ovarian cancer cell lines (IGROV-1 and SKOV-3) were incubated in the presence of a Wnt inhibitor ICG-001). The cells were incubated in the presence of ICG-001: 1, 10, 20 and 50 µM or with no stimulus (control). After 48hs of incubation, we harvested the cells to count them and we performed protein extracts for western blot technique. We also determined apoptosis by citometry: cells were double-stained with Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI).Our results showed a substantial and dose dependent decrease in proliferation when both cell lines were incubated in the presence of ICG-001. We also observed a decrease in protein levels of Wnt/β-catenin and CyclinD1, when cells were treated with the inhibitor (with all the concentration used). Regarding to apoptosis, we observed a low percentage of apoptotic cells ( 25%) in cells incubated with 20 and 50 µM ICG-001.In conclusion, we demonstrate that Wnt/β-catenin signalling regulates ovarian cancer cell lines proliferation and apoptosis.