ANTI-ADIPOGENIC EFFECT OF THE ANTIOXIDANT N-ACETYLCYSTEINE ON 3T3-L1 ADIPOCYTES

DI MARZI M; GABRIELLI M; PIERALISI A; GUERRA LN; MITAROTONDA R; MARTINI CN; CALVO JC; VILA MC; SOTO D

Congreso; SOCIEDADES DE BIOCIENCIAS. LXII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC).; 2017

Reports about antioxidant effect on obesity are controversial. We showed that N-acetylcysteine (NAC) inhibits cellular lipid accumulationduring 3T3-L1 adipocyte differentiation, through the inhibition of adipogenictranscription factors expression, such as PPARã and, MAPK phosphorylation (Soto el al, Redox Rep 2016). Objectives: Here, we evaluated NAC effect on fully differentiated 3T3-L1 adipocytes (AC) and developed nanoparticles with NAC. Methods: Assays were conducted using AC, different doses of NAC (0.01 to 5 mM) were added to culture media for 5 days and, we determined: cellular content of triglycerides (Tg) and Oil-Red-O stained lipids(ORO), NAC toxicity by MTT assay, PPARã protein expression by western blot assay, mRNA levels of PPARã and lipid protein perilipin (Pl) y RT-qPCRanalysis. NAC content in nanoparticles was determined by Raggi method. Results: Treatments with 0.01 to 5 mM NAC were not toxic on AC. 5mM NAC treatment on AC (ACN) elicited a 60% decrease in cellular Tg content (1.22+0.09 gTg/g protein[AC] vs 0.49+0.03 gTg/g protein [ACN], p